Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

Artificial insemination using sexing semen is expected to produce calves with the desired sex. One sperm method percoll density gradient centrifugation method. This study aimed determine changes and relationship between motility acrosome reaction after process centrifugation. The material used was of ±5 years old Ongole crossbred bull a bodyweight ±700 kg as many three bulls mass 2+ individual 70%. compare fresh sexed cooling process. Parameters measured were characters CASA analysis, which included parameters, progressive motility, capacitation, no reaction. Statistical analysis paired T-test distinguish among semen, In comparison, regression correlation analyze capacitation hyperactivation acrosomal motility. results showed that decreased Meanwhile, reaction, hyperactivity increased.